Padhi Research Group
Biocatalysis and Enzyme Engineering Laboratory
Department of Biochemistry
School of Lifesciences
Hydroxynitrile lyase engineering for promiscuous asymmetric Henry reaction with enhanced conversion, enantioselectivity and catalytic efficiency
Badipatla Vishnu Priya, D. H. Sreenivasa Rao, Ayon Chatterjee, Santosh Kumar Padhi
Chem. Commun., 2023, DOI: 10.1039/D3CC02837B.
Biochemical characterization, substrate and stereoselectivity of an outer surface putative α/β hydrolase from the pathogenic Leptospira
Umate Nachiket Shankar, Mohit, Santosh Kumar Padhi, Mohd. Akif
Int. J. Biol. Macromol. 2023, In Press, https://doi.org/10.1016/j.ijbiomac.2022.12.283
Biocatalytic approaches for enantio and diastereoselective synthesis of chiral β-nitroalcohols
D.H. Sreenivasa Rao, Ayon Chatterjee, Santosh Kumar Padhi
Org. Biomol. Chem., 2021, 19, 322-337, https://doi.org/10.1039/D0OB02019B
Immobilized Arabidopsis thaliana hydroxynitrile lyase catalyzed retro-Henry reaction in the synthesis of (S)-β-nitro alcohols
D.H. Sreenivasa Rao, Kummari Shivani, Santosh Kumar Padhi
Appl. Biochem. Biotechnol, 2021, 193, 560-576, https://doi.org/10.1007/s12010-020-03442-3
An ultrasensitive fluorescence assay for detection of halides and enzymatic dehalogenation
AÅŸkın S. Aslan-Üzel, Andy Beier, David KováÅ™, Clemens Cziegler, Santosh K. Padhi, Mark Dörr, Dominique Böttcher, Frank Hollmann, Florian Rudroff, Marko D. Mihovilovic, Tomáš Buryška, JiÅ™í Damborský, ZbynÄ›k Prokop, Christoffel P. S. Badenhorst, Uwe T. Bornscheuer
ChemCatChem., 2020, 12, 2032-2039 https://doi.org/10.1002/cctc.201901891
A study on increasing enzymatic stability and activity of Baliospermum montanum hydroxynitrile lyase in biocatalysis
Nisha Jangir, Preeti, Santosh Kumar Padhi
Process Biochem., 2020, 88, 78-89, https://doi.org/10.1016/j.procbio.2019.10.014
Production of (S)-β-nitro alcohols by enantioselective C-C bond cleavage with an (R)-selective Hydroxynitrile lyase
D.H. Sreenivasa Rao, Santosh Kumar Padhi
ChemBioChem. 2019, 20, 371-378, https://doi.org/10.1002/cbic.201800416
Baliospermum montanum hydroxynitrile lyase catalyzed synthesis of chiral cyanohydrins in a biphasic solvent
Nisha Jangir, Dheeraj Sangoji, and Santosh Kumar Padhi
Biocatal Agric Biotechnol., 2018, 16, 229-236, https://doi.org/10.1016/j.bcab.2018.08.008
Modern approaches to discovering new hydroxynitrile lyases for biocatalysis
Santosh Kumar Padhi
ChemBioChem, 2017, 18, 152-160, https://doi.org/10.1002/cbic.201600495
Candida parapsilosis: A versatile biocatalyst for organic oxidation-reduction reactions
Anju Chadha, Sowmyalakshmi Venkataraman, Radhakrishnan Preetha, Santosh Kumar Padhi
Bioorg. Chem., 2016, 68, 187-213, https://doi.org/10.1016/j.bioorg.2016.08.007
Publications before joining UoH
14. David Nedrud, Hui Lin, Gilsinia Lopez, Santosh K. Padhi, Graig A. Legatt, Romas Kazlauskas, Uncovering divergent evolution of α/β-hydrolases: a surprising residue substitution needed to convert Heavea brasiliensis hydroxynitrile lyase into an esterase. Chemical Science, 2014, 5, 4265-4277.
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13. Henrike brundiek, Santosh Kumar Padhi, Robert kourist, Andrew Evitt, and Uwe T. Bornscheuer, Altering the scissile fatty acid binding site of Candia antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids. Eur. J. Lipid Sci. Tech., 2012, 114, 1148-1153.
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12. Santosh Kumar Padhi, Michael Haas, and Uwe T Bornscheuer, Lipase catalyzed transesterification to remove saturated monoglycerides from biodiesel. Eur. J. Lipid Sci. Tech., 2012, 114, 875-879.
11. Geoffrey A. Behrens, Anke Hummel, Santosh K. Padhi, Sebastian Schätzle and Uwe T Bornscheuer, Discovery and protein engineering of biocatalysts for organic synthesis. Adv. Synth. Catal. 2011, 353, 2191-2215.
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10. Helge Jochens, Martin Hesseler, Konstanz Stiba, Santosh Kumar Padhi, Romas J Kazlauskas and Uwe T Bornscheuer, Protein engineering of α/β hydrolase fold enzymes. ChemBioChem. 2011, 12, 1508-1517.
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9. Santosh Kumar Padhi, Ryota Fujii, Graig Legatt, Sara Fossum, Reto Berchtold and Romas J Kazlauskas, Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions. Chem. Biol. 2010, 17,863-871.
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8. Robert Kourist, Helge Jochens, Sebastian Bartsch, Remko Kuipers, Santosh Kumar Padhi, Markus Gall, Dominique Bottcher, Henk-Jan Joosten and Uwe T Bornscheuer, The α/β hydrolase fold 3DM database (ABHDB) as a tool for protein engineering. ChemBioChem. 2010, 11, 1635-1643.
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7. Santosh Kumar Padhi, Despina Boughkou and Jon D Stewart, Site saturation mutagenesis of tryptophan 116 of Saccharomyces pastorianus old yellow enzyme uncovers stereocomplementary variants. J. Am. Chem. Soc. 2009, 131, 3271-3280.
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6. Santosh Kumar Padhi, Iwona A Kaluzna, Didier Buisson, Robert Azerad and Jon D Stewart, Reductions of cyclic β-keto esters by individual Saccharomyces cerevisiae dehydrogenases and a chemo-enzymatic route to (1R,2S)-2-methyl-1-cyclohexanol. Tetrahedron Asymmetry, 2007, 18, 2133-2138.
5. Santosh Kumar Padhi, D. Titu, N. Ganesh Pandian and Anju Chadha, Deracemisation of b-hydroxy esters using immobilised whole cells of Candida parapsilosis ATCC 7330: Substrate specificity and mechanistic investigation. Tetrahedron, 2006, 62, 5133-5140.
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4. Santosh Kumar Padhi and Anju Chadha, Deracemisation of aromatic b-hydroxy esters using immobilised whole cells of Candida parapsilosis ATCC 7330 and determination of absolute configuration by 1H NMR. Tetrahedron Asymmetry, 2005, 16, 2790-2798.
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3. Santosh Kumar Padhi, N. Ganesh Pandian and Anju Chadha, Microbial deracemisation of aromatic b-hydroxy acid esters. J. Mol. Catal. B. Enzym. 2004, 29, 25-29.
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2. Santosh Kumar Padhi, N. Ganesh Pandian and Anju Chadha, Microbial deracemisation of β-hydroxy esters - An important strategy towards various chiral intermediates. Chem. Listy 2003, 97,479-480.
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1. Santosh Kumar Padhi and Anju Chadha, Sodium borohydride reduction and selective transesterification of b- keto esters in one pot reaction. Synlett, 2003, 05, 639-642.